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April 7, 2023jessica-sacher
Phage methods Seeking suggestions

Hello Phage People,
Does anyone use Phage DNA Isolation Kit from Norgen Biotek? I’m using this kit and trying to increase the DNA concentration. I already did all the additional steps but does anyone have any tricks for increasing concentration? I appreciate it if you mail me your tricks.
Best, Irem

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aw ∙ April 29, 2023
Maybe not relevant, but just sharing my opinion. I once had to isolate about 1-2 µg of phage DNA. My approach is to do a CsCL centrifuge and then extract from the concentrated phage band. best, AW